Plasmid structure

(a) Selected genes important in donor–recipient cell conjugation and F factor transfer are shown along with the origin of transfer (oriT) and four insertion sequences (IS) around the 100-kb map of the F plasmid of the E. coli K-12 strain. (b) The 36-base sequence of oriT, including the cleavage site on the T strand(a) Selected genes important in donor–recipient cell conjugation and F factor transfer are shown along with the origin of transfer (oriT) and four insertion sequences (IS) around the 100-kb map of the F plasmid of the E. coli K-12 strain. (b) The 36-base sequence of oriT, including the cleavage site on the T strand

(a) F factor map Relaxosome (b) oriT strand sequence Base pairs 1 1O 20 30 5’ CCA GTT TCT CGA AGA AAC CGG TAA ATG CGC CCT CCC 3’ T Cleavaqe site