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Step 2 of gene cloning: The actions of a restriction enzyme and DNA ligase to produce a recombinant

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Step, 2, gene, cloning:, actions, restriction, enzyme, DNA, ligase, produce, recombinant,
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The restriction enzyme binds to a specific sequence in both the chromosomal and the vector DNA. It then cleaves the DNA backbones, producing DNA fragments. The complementary single-stranded ends of the DNA fragments hydrogen bond with each other. The enzyme DNA ligase then covalently links these fragments, producing a recombinant vector.
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