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In a typical proteomic analysis, cells are exposed to different conditions

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21–22 In a typical proteomic analysis, cells are exposed to different conditions (such as different growth conditions, drugs, or hormones

Mass (kDa) Cells grown in vitro i Condition A Condition B Exlracl proleins Exiracl proleins 2DGE 2DGE 200 200 o o o E 5 - o a - M E - ; O 4.0 pH —> 10.0 Compare H Expression pallerns No expression Cut spat out i Mass spectrometry and comparison to prolein databases to identify the prolein in the spot.
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