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Guide RNA (gRNA) directs RNA editing
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Description
5'Q_, AAAAGGCTTTAA “7‘3’Codingstrand - 5’ Template strand | Transcription l mRNA 5’—3’ | Pairing with guide RNA l Single-stranded guide RNA pairs with a portion of messenger RNA. Note adenine nucleotides in unpaired loops. mRNA 5'— 3' gRNA 3' DNA RNA editing l Nuclease enzyme cuts mRNA, and RNA polymerase uses unpaired adenines of guide RNA to add uracils to mRNA. Release of edited mRNA l RNA-edited mature mRNA contains uracil nucleotides not encoded by DNA. mRNA 5'—3'
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