An example of transduction by P1 phage

Transducing phages are generated by the mistaken packaging of a fragment of the donor bacterium’s DNA into a phage head. Transductant bacteria are produced by homologous recombination between the introduced fragment of donor DNA and the recipient bacterial chromosomeTransducing phages are generated by the mistaken packaging of a fragment of the donor bacterium’s DNA into a phage head. Transductant bacteria are produced by homologous recombination between the introduced fragment of donor DNA and the recipient bacterial chromosome

Phage P1 0 P1 phage infects 3 met‘, his’ donor cell. _ P1 /Donor bacterium DNA his’ met' (meta hIS') Bacterial chromosome of bacterial Fragments l chromosome 0 Phage chromosome is replicated, and phage proteins are expressed. The donor chromosome fragments. 9 Progeny phage assembly yields normal phage carrying the phage chromosome and transducing phages carrying a fragment of the donor chromosome. Progeny P1 phage 0 Lysis releases normal and ‘ transducing progeny phages. 9 A met‘ transducing phage infects a met‘ recipient cell and injects the donor DNA fragment. hiS‘ _ Recipient bacterium met / (met , his) Bacterial chromosome @ Homologous recombination at two crossover points exchanges segments between the donor fragment and the recipient chromosome. 0 The transductant is met', his: Excised DNA containing mat is degraded. Transductant bacterium (met‘, his)