Yanofsky’s cotransduction frequency analysis

Yanofsky’s cotransduction frequency analysis and mapping of trp operon genes in E. coli. (a) Cotransduction frequencies of cys+ and a gene of the trp operon are determined in separate selected marker–unselected marker experiments. (b) Yanofsky’s proposed map of the trp operonYanofsky’s cotransduction frequency analysis and mapping of trp operon genes in E. coli. (a) Cotransduction frequencies of cys+ and a gene of the trp operon are determined in separate selected marker–unselected marker experiments. (b) Yanofsky’s proposed map of the trp operon

(a) Cotransduction frequencies Percent cotransduction Donor Recipient Selected Unselected 01‘ unselected genotype genotype marker marker marker With CyS+ cys+ trpE+ cy5’ trpE‘ cyst trpE+ 63 cys+ trpC+ cy5" trpC‘ cys+ trpC+ 53 cys+ trpB+ cyS‘ trpB“ cys+ trpB+ 47 cys+ trpA+ cyr trpA‘ cys+ trpA+ 46 (b) trp operon map III-III: cys trpE trpC trpB trpA