Biochemistry

In prokaryotic replication, all DNA polymerases are capable of all of the following tasks except: ...

In prokaryotic replication, all DNA polymerases are capable of all of the following tasks except: ...

In prokaryotic replication, all DNA polymerases are capable of all of the following tasks except:
 A) Reading a template strand and putting the complementary base in the correct position
  B) Polymerizing DNA in the 5' to 3' direction

Miller123   45   2 Replies

Miller123

In E. coli, : (Multiple Choice)

In E. coli, : (Multiple Choice)

In E. coli,
 A) the leading strand is synthesized in one piece while the lagging strand is synthesized discontinuously.
  B) the leading strand is synthesized discontinuously while the lagging strand is synthesized in one piece.
  C

alexiswells   77   1 Reply

Mulatta

AZT, used in treating AIDS, is an effective inhibitor of nucleic acid synthesis because:

AZT, used in treating AIDS, is an effective inhibitor of nucleic acid synthesis because:

AZT, used in treating AIDS, is an effective inhibitor of nucleic acid synthesis because:
 A) It has a nitrogen group instead of a 3' OH.
  B) It is a 2', 3' dideoxy compound.
  C) It doesn't form good base pairs.
  D) It inhib

lacinb30   52   1 Reply

rayohn2010

Which of the following activities does E. coli DNA polymerase III lack?

Which of the following activities does E. coli DNA polymerase III lack?

Which of the following activities does E. coli DNA polymerase III lack?
 A) 5'  3' polymerase
  B) 5'  3' exonuclease
  C) 3'  5' exonuclease
  D) E. coli DNA polymerase III has ALL of the above activities.

hiufwsiuefbwe   58   1 Reply

taylorhovland

The primer for in vivo DNA replication is:

The primer for in vivo DNA replication is:

The primer for in vivo DNA replication is:
 A) The 3' hydroxyl of the preceding Okazaki fragment.
  B) A short piece of RNA.
  C) A nick made in the DNA template.
  D) A primer is not always required for DNA replication.

linasottile   73   1 Reply

achurch

The energetic driving force for nucleic acid synthesis is :

The energetic driving force for nucleic acid synthesis is :

The energetic driving force for nucleic acid synthesis is
 A) removal of pyrophosphate from the incoming nucleotide.
  B) removal of pyrophosphate from the growing strand.
  C) removal of inorganic phosphate from the incoming nucleo

jayfro   63   1 Reply

garroghuu

E. coli replication on the lagging strand :

E. coli replication on the lagging strand :

E. coli replication on the lagging strand
 A) is carried out by DNA polymerase I
  B) is initially synthesized as Okazaki fragments
  C) is synthesized continuously
  D) has this DNA strand synthesized in a 3'  5' direction

adamsnaya   51   2 Replies

adamsnaya

Which of the following is not a function of DNA polymerase I from E. coli?

Which of the following is not a function of DNA polymerase I from E. coli?

Which of the following is not a function of DNA polymerase I from E. coli?
 A) adding nucleotides to the primer strand
  B) 3'  5' exonuclease activity
  C) 5'  3' exonuclease activity
  D) proofreading

lovelyturret   61   1 Reply

car2065596

In order to replicate both strands, E. coli :

In order to replicate both strands, E. coli :

In order to replicate both strands, E. coli
 A) displaces one of the strands and folds it back so both strands face in the same direction, so they can be synthesized at once.
  B) synthesizes each strand in the opposite direction (one 5'

Jnortontruvisio   48   1 Reply

salmajed1

DNA polymerase III from E. coli :

DNA polymerase III from E. coli :

DNA polymerase III from E. coli
 A) is a large, multisubunit protein
  B) has a sliding clamp portion that anchors it to DNA
  C) has a polymerization site separate from the DNA binding site
  D) all of these

Griffinw42   40   1 Reply

elizabethxo1

The replisome in prokaryotic replication consists of:

The replisome in prokaryotic replication consists of:

The replisome in prokaryotic replication consists of:
 A) a protein dimer
  B) a protein tetramer
  C) a multisubunit complex of at least 13 different proteins
  D) more than 100 separate proteins

stalmoney   46   1 Reply

Beehappy2

The mechanism for all template-directed synthesis of any type of nucleic acid involves the ...

The mechanism for all template-directed synthesis of any type of nucleic acid involves the ...

The mechanism for all template-directed synthesis of any type of nucleic acid involves the following:
 A) Nucleophilic attack of a 3' hydroxyl toward a nucleoside triphosphate, releasing PPi.
  B) Nucleophilic attack of a 5' hydroxyl towa

alshernandez   59   1 Reply

garroghuu

Okazaki fragments are : (Multiple Choice)

Okazaki fragments are : (Multiple Choice)

Okazaki fragments are
 A) short DNA pieces that explain how DNA is synthesized on the lagging strand.
  B) short DNA pieces that explain how DNA is synthesized on the leading strand.
  C) the remnants of the original strands that ar

lacinb30   49   2 Replies

lacinb30

At the replisome of prokaryotic replication :

At the replisome of prokaryotic replication :

At the replisome of prokaryotic replication
 A) there are only two DNA Polymerase III molecules
  B) There are multiple copies of DNA Polymerase I
  C) Two molecules of Polymerase III are dedicated to lagging strand synthesis while

linasottile   59   1 Reply

Lettyyys

A prokaryotic replisome typically contains three molecules of DNA pol III, but only one molecule of ...

A prokaryotic replisome typically contains three molecules of DNA pol III, but only one molecule of ...

A prokaryotic replisome typically contains three molecules of DNA pol III, but only one molecule of DNA pol I. Why?
 A) The DNA pol I works on the leading strand, while DNA pol IIIs work on the Okazaki fragments. since there are several of thos

tarapratt95   33   1 Reply

Godzilla159

In the synthesis of DNA in Escherichia coli :

In the synthesis of DNA in Escherichia coli :

In the synthesis of DNA in Escherichia coli
 A) a single-strand nick in the template DNA gives rise to a swivel point just in advance of the replication fork
  B) Mn2+ is required
  C) all enzymes involved are single polypeptide cha

jayfro   60   1 Reply

gidgetlynch@gma

What is the need for a primer strand in DNA replication?

What is the need for a primer strand in DNA replication?

What is the need for a primer strand in DNA replication?
 A) it ensures the fidelity of the newly synthesized DNA strand
  B) the DNA polymerases require a preexisting strand with a nucleotide having a 3'OH
  C) the DNA polymerases

Griffinw42   39   1 Reply

bunique227744

In Escherichia coli, the enzyme principally responsible for the synthesis of new DNA strands is :

In Escherichia coli, the enzyme principally responsible for the synthesis of new DNA strands is :

In Escherichia coli, the enzyme principally responsible for the synthesis of new DNA strands is
 A) DNA polymerase I
  B) DNA polymerase III
  C) DNA ligase
  D) primase

ksultz   61   1 Reply

Lettyyys

Which of the following is true about clamp loaders?

Which of the following is true about clamp loaders?

Which of the following is true about clamp loaders?
 A) They are pentameric enzymes
  B) They serve to open up the sliding clamp and insert the DNA
  C) They are ATPases
  D) All of these

ugai_kyut   32   2 Replies

ugai_kyut

Which of the following characteristics is not associated with E. coli primase?

Which of the following characteristics is not associated with E. coli primase?

Which of the following characteristics is not associated with E. coli primase?
 A) it synthesizes the RNA primer in DNA replication
  B) it synthesizes a primer with a free 3'OH end
  C) it is essential for DNA replication
  D

bronzeclock   65   1 Reply

hollyhateschool

Why is thymine used in DNA rather than uracil?

Why is thymine used in DNA rather than uracil?

Why is thymine used in DNA rather than uracil?
 A) Thymine is more hydrophobic, so it stacks better in the helix.
  B) If cytosine is deaminated, it forms uracil, which can be easily distinguished from thymine.
  C) Thymine is not c

adamsnaya   52   1 Reply

John Ross

Which of these proteins is used less for leading strand replication than for lagging strand ...

Which of these proteins is used less for leading strand replication than for lagging strand ...

Which of these proteins is used less for leading strand replication than for lagging strand replication?
 A) DNA polymerase
  B) DNA-binding protein
  C) DNA ligase
  D) DNA gyrase

Greenspotsock   88   1 Reply

bunique227744

Which of the following is not required for optimal DNA replication?

Which of the following is not required for optimal DNA replication?

Which of the following is not required for optimal DNA replication?
 A) Primase
  B) DNA Polymerase II.
  C) Single strand binding proteins.
  D) Gyrase
  E) All of these are necessary.

lovelyturret   75   1 Reply

mousesareplural

Which of the activities of DNA Polymerase I is most important in its role of proofreading?

Which of the activities of DNA Polymerase I is most important in its role of proofreading?

Which of the activities of DNA Polymerase I is most important in its role of proofreading?
 A) Polymerase activity.
  B) Ability to nick intact double stranded DNA.
  C) 5'  3' exonuclease.
  D) 3'  5' exonuclease.
  E)

ksultz   38   1 Reply

Findingskate61

Which is most likely to have a high processivity?

Which is most likely to have a high processivity?

Which is most likely to have a high processivity?
 A) a DNA polymerase that is primarily a repair enzyme
  B) a primase
  C) a DNA ligase
  D) a DNA polymerase that is a main polymerizing enzyme

hawkins1346   45   2 Replies

hawkins1346

The enzyme that attaches the Okazaki fragments together is called :

The enzyme that attaches the Okazaki fragments together is called :

The enzyme that attaches the Okazaki fragments together is called
 A) ligase.
  B) primase.
  C) DNA polymerase I
  D) DNA polymerase III

shoppa   76   1 Reply

Keeze

Which of the activities of DNA Polymerase I is most important in removing the primer?

Which of the activities of DNA Polymerase I is most important in removing the primer?

Which of the activities of DNA Polymerase I is most important in removing the primer?
 A) Polymerase activity.
  B) Ability to nick intact double stranded DNA.
  C) 5'  3' exonuclease.
  D) 3'  5' exonuclease.
  E) None

Jnortontruvisio   93   1 Reply

physico

Single strand binding proteins are important for this activity:

Single strand binding proteins are important for this activity:

Single strand binding proteins are important for this activity:
 A) Prevent single-stranded DNA from rewinding.
  B) Protect single-stranded DNA from enzymatic degradation.
  C) Prevent double helical DNA from unwinding.
  D)

stalmoney   90   1 Reply

pripaw

Which of the following is not an activity of DNA Polymerase I?

Which of the following is not an activity of DNA Polymerase I?

Which of the following is not an activity of DNA Polymerase I?
 A) Polymerase activity.
  B) Ability to nick intact double stranded DNA.
  C) 5'  3' exonuclease.
  D) 3'  5' exonuclease.
  E) All of these are present in

Griffinw42   39   1 Reply

MSROGERS

Which of the following repair mechanisms would most likely be used to repair a G in DNA damaged by ...

Which of the following repair mechanisms would most likely be used to repair a G in DNA damaged by ...

Which of the following repair mechanisms would most likely be used to repair a G in DNA damaged by oxidation?
 A) Base Excision:
  B) Nucleotide Excision
  C) Pol III Proofreading
  D) Mismatch Repair

rachelkimq   65   1 Reply

mrperretti

The proofreading of DNA is especially good because the identity of each base pair is checked before ...

The proofreading of DNA is especially good because the identity of each base pair is checked before ...

The proofreading of DNA is especially good because the identity of each base pair is checked before the enzyme moves on to the next base pair.
 A) True
  B) False

stalmoney   34   2 Replies

stalmoney

DNA repair mechanisms are essentially the same whether the DNA has minor damage or major damage. :

DNA repair mechanisms are essentially the same whether the DNA has minor damage or major damage. :

DNA repair mechanisms are essentially the same whether the DNA has minor damage or major damage.
 A) True
  B) False

jayfro   56   1 Reply

ravindergill185

It is so important to keep the DNA molecule fully connected that some repair mechanisms will ...

It is so important to keep the DNA molecule fully connected that some repair mechanisms will ...

It is so important to keep the DNA molecule fully connected that some repair mechanisms will actually allow mismatches or deletions from the DNA.
 A) True
  B) False

jennjenn563   24   1 Reply

rayohn2010

Ultra-violet light principally causes which of the following damages to DNA?

Ultra-violet light principally causes which of the following damages to DNA?

Ultra-violet light principally causes which of the following damages to DNA?
 A) mismatches between stands
  B) breaks in the phosphodiester backbone of the DNA strand
  C) thymine dimerization
  D) methylation of specific bas

Jnortontruvisio   26   1 Reply

bunique227744

Since DNA Polymerase II has endonuclease activity, it is able to proofread its product when it is ...

Since DNA Polymerase II has endonuclease activity, it is able to proofread its product when it is ...

Since DNA Polymerase II has endonuclease activity, it is able to proofread its product when it is used in DNA repair.
 A) True
  B) False

rachelkimq   34   1 Reply

131231

Which of the following enzymes is used to create AP sites?

Which of the following enzymes is used to create AP sites?

Which of the following enzymes is used to create AP sites?
 A) primase
  B) helicase
  C) ligase
  D) glycosylase

kekebab001   38   1 Reply

yuhiyuhi

Which repair mechanism has an intermediate wherein a region of single-stranded DNA is temporarily ...

Which repair mechanism has an intermediate wherein a region of single-stranded DNA is temporarily ...

Which repair mechanism has an intermediate wherein a region of single-stranded DNA is temporarily created?
 A) Base Excision
  B) Nucleotide Excision
  C) Pol III Proofreading
  D) Mismatch Repair

redheadprincess   20   2 Replies

redheadprincess

One of the most important ways in which DNA is modified after synthesis is :

One of the most important ways in which DNA is modified after synthesis is :

One of the most important ways in which DNA is modified after synthesis is
 A) methylation of bases
  B) covalent binding of proteins to the sugar moieties
  C) splicing of RNA leaders
  D) electrostatic binding of negatively

jennjenn563   33   1 Reply

cotdiyea

Which of the following mechanisms allow repair enzymes to distinguish incorrect bases in DNA?

Which of the following mechanisms allow repair enzymes to distinguish incorrect bases in DNA?

Which of the following mechanisms allow repair enzymes to distinguish incorrect bases in DNA?
 A) The newly synthesized strand of DNA lacks methylated bases and other modifications.
  B) Deamination of A and C lead to improper bases for D

adamsnaya   30   1 Reply

Keeze

Which of the following statements is true about the so-called MUT (for mutation) repair mechanisms? ...

Which of the following statements is true about the so-called MUT (for mutation) repair mechanisms? ...

Which of the following statements is true about the so-called MUT (for mutation) repair mechanisms?
 A) Depend on the methylation of old strands of DNA to determine which strand to repair.
  B) Often remove hundreds or even thousands of b

ksultz   25   1 Reply

bunique227744