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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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michelle6899 michelle6899
wrote...
6 years ago
In E. coli, how are the Okazaki fragments of the lagging strands joined to create a complete DNA duplex?
 
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#1
6 years ago
Ans: Each Okazaki fragment has an RNA sequence at the 5 end and a free 3-OH end adjacent to the 5 end of the next fragment. In E. coli, DNA polymerase I replaces the RNA primer with DNA. The nicks between fragments are resolved by the enzyme DNA ligase, which joins the 3-OH end of one fragment to the 5-phosphate end of the next fragment.
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