Some members of the species D. bifasciata produce mostly female offspring if reared at or below 21C. ...

Some members of the species D. bifasciata produce mostly female offspring if reared at or below 21C. The causative agent of this condition (called sex ratio) is a protozoan infection that is lethal to most male larvae.
 
  How can you demonstrate that it is both inherited and extrachromosomal? A) Inject ooplasm of affected females into normal females and then mate these with normal males.
  B) Inject cytoplasm from somatic cells of an affected female into normal females and then mate them with normal males.
  C) Mate affected females with normal males and assess daughter somatic cells for presence of the protozoan.
  D) Perform reciprocal crosses of affected and normal individuals and examine the offspring for infection.
  E) Perform reciprocal crosses of affected and normal individuals and look for affected male offspring.