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dero dero
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11 years ago
I'm in a Biological Physics class and we have a requirement to write a summary paper on a journal submission of our choosing.

The paper: Identification of a Plant Receptor for Extracellular ATP (Jeongmin Choi et al. Science 343, 290 (2014)

There is one part that lost me pretty quickly:

"The identification of the LecRK-I.9 gene allowed
isolation of a dorn1 transferred DNA (T-DNA) insertion
(Arabidopsis Biological Resource Center
accession code Salk_042209; dorn1-3), which has
reduced transcript levels (fig. S9). The phenotype
of the dorn1-3 mutant was identical to that of
dorn1-1 and dorn1-2 in that the mutant did not
show an increase in intracellular calcium concentration
or gene induction upon the addition of
ATP (Fig. 1A and fig. S10)."

I'm not sure if context is really required or not but the first sentence is where I'm truly lost. Are they saying they basically transferred the dorn1 genes into a new dorn1-3 plant successfully?

Thanks for any calirfication provided!
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wrote...
Staff Member
Educator
11 years ago
HI

The phenotype of the dorn1-3 mutant was identical to that of dorn1-1 and dorn1-2 in that the mutant did not show an increase in intracellular calcium concentration or gene induction upon the addition of ATP (Fig. 1A and fig. S10)."

In simpler terms, the physical traits presented by the dorn1-3 mutant were identical to those found in dorn1-1 and dorn1-2.
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dero Author
wrote...
11 years ago
I understood that they ended up being identical. My question is if T-DNA is just means that dorn1-1 DNA was placed into another plant in order to replicate their results?
wrote...
Staff Member
Educator
11 years ago
"The identification of the LecRK-I.9 gene allowed isolation of a dorn1 transferred DNA (T-DNA) insertion (Arabidopsis Biological Resource Center accession code Salk_042209; dorn1-3), which has reduced transcript levels (fig. S9).

By identifying the LecRK-I.9 gene sequence, they were able to isolate the dorn1 gene for the purpose of transferring it.
Mastering in Nutritional Biology
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