Explain the role of trypsin in identifying the primary structure of a protein. What will be an ...

ANSWER: Trypsin is a digestive enzyme that is used to produce polypeptide fragments useful for sequence analysis. Trypsin is the most commonly used reagent for specific proteolysis. Trypsin will only hydrolyze peptide bonds in which the carbonyl function is contributed by an arginine or a lysine residue. That is, trypsin cleaves on the C-side of Arginine (Arg) or Lysine (Lys), generating a set of peptide fragments having Arg or Lys at their C-termini. The number of smaller peptides resulting from trypsin action is equal to the total number of Arg and Lys residues in the protein plus onethe protein's C-terminal peptide fragment.