A wild-type culture of haploid yeast is exposed to ethyl methanesulfonate (EMS). Yeast cells are plated on a complete medium, and 6 colonies (colonies numbered 1 to 6) are transferred to a new complete medium plate for further study. Four replica plates are made from the complete medium plate to plates containing minimal medium or minimal medium plus one amino acid (replica plates numbered 1 to 4) with the following results:
Identify the colonies that are prototrophic (wild type).
Check all that apply.1
2
3
4
5
6
Part B
Identify the colonies that are auxotrophic (mutant).
Check all that apply.1
2
3
4
5
6
Part C
Identify any colonies that are His-, Arg-, Leu-
Drag the appropriate items to their respective bins.Colonies: 1, 2, 3, 4, 5, 6
Bins: His- Arg- Leu- Other Colonies
Part D
Choose the appropriate genotypes for the following colonies, (choose "+" for the wild-type synthesis and " -" for the mutant synthesis of arginine, histidine, and leucine).
Drag the appropriate items to their respective bins.Arg+His+Leu+ Arg+His-Leu+ Arg+His+Leu-
Colony1 Cololny 3 Colony 5
Are there any colonies for which genotype information cannot be determined? If so, which colony or colonies?
Check all that apply.1
2
3
4
5
6
There are no such colonies.
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