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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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alijan99 alijan99
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6 years ago
In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya be able to grow on medium that contains lactose as the sole carbon source? Why or why not?
 
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Genetic Analysis: An Integrated Approach

Genetic Analysis: An Integrated Approach


Edition: 3rd
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#1
6 years ago
No. Metabolically, cells that are cya would be similar to wild-type cells that are grown in the presence of both glucose and lactose. Although negative regulation has been relieved (the small amounts of allolactose produced binds the lac repressor protein, removing it from the lac operator), there is no positive regulation by CAP-cAMP because cAMP is not synthesized. Therefore, only basal transcription occurs. This is not sufficient to drive active lactose metabolism.
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