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boobear86 boobear86
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Posts: 640
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6 years ago
A prokaryote that differs from most other bacteria with respect to its very small size and obligates intracellular parasitism is:
 
  a. Rickettsia.
  b. Bartonella.
  c. Calymmatobacterium.
  d. Coxiella.



To isolate anaerobic gram-positive organisms, which of the following media should be used?
 
  a. Anaerobic blood agar
  b. Cycloserine cefoxitin fructose agar (CCFA)
  c. Laked kanamycin-vancomycin (LKV) blood agar
  d. Anaerobic phenylethyl alcohol agar (PEA)



Which organism may be mistaken for a lactose fermenter on MacConkey agar because of colonies exhibiting a purple hue on the media?
 
  a. Acinetobacter spp.
  b. P. luteola
  c. S. maltophilia
  d. Bordetella holmesii



The term complex as used in the Mycobacterium tuberculosis complex:
 
  a. means that the identification of the organ-ism is complex and is only performed in large reference laboratories.
  b. is used to describe two or more species whose distinction is complicated and of little or no medical importance.
  c. refers to a set of organisms that are genet-ically indistinguishable but are phenotyp-ically different.
  d. refers to different subtypes of the bacteria M. tuberculosis.



Quality control is set up to test urea agar. M. genitalium and M. hominis are used; however, both organisms are negative for urease activity. Which one of the following statements explains these findings?
 
  a. The urea agar is most likely expired.
  b. The incubation time is most likely abbre-viated.
  c. A technical error has occurred.
  d. These two organisms are poor choices for quality control of urea agar.
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Replies
wrote...
6 years ago
A
The organisms addressed in Chapter 43 are obligate intracellular bacteria or are considered either extremely difficult to culture or unable to be cultured. Organisms of the genera Chlamydia, Chlamydophila, Rickettsia, Orientia, Anaplasma, and Ehrlichia are prokaryotes that differ from most other bacteria with respect to their very small size and obligate intracellular parasitism.

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D
Anaerobic PEA is similar to aerobic PEA in that it is selective for gram-positive bacteria but in-hibits enteric gram-negative rods and swarming by some Clostridia.

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A
Although a nonlactose fermenter, Acinetobacter spp. produces colonies that exhibit a purplish hue and may cause the organism to be mistaken for a lactose fermenter. The other organisms are nonlactose fermenters as well; however, they do not demonstrate this characteristic.

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B
The term complex is used in the clinical microbiology laboratory setting to describe two or more species whose distinction is complicated and of little or no medical importance. The mycobac-terial species that occur in humans and belong to the M. tuberculosis complex include M. tuber-culosis, Mycobacterium bovis, M. bovis bacillus Calmette-Gurin (BCG), Mycobacterium afri-canum, Mycobacterium caprae, Mycobacterium microti, Mycobacterium canettii, and Mycobac-terium pinnipedii. All of the species in the M. tuberculosis complex are capable of causing tu-berculosis.

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D
Both M. genitalium and M. hominis are negative for urease activity; therefore they are poor choices to test the validity of the agar. Either one of these organisms, along with U. urealyticum, should have been used; they are both negative and positive for urease activity, respectively.
boobear86 Author
wrote...
6 years ago
This is very helpful, my teacher this year is not good
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