What kind of an inhibitor is Prozac?

shelby88

wrote...

13 years ago

I need help drawing the Linweaver-Burke plot for this. My graph doesn't look right when I did it. Anybody?

Also, to answer these questions:

-what kind of an inhibitor is Prozac? my answer was uncompetitive inhibitor? is that right?
What is Km for the enzyme with the inhibitor? since my graph turned out wrong, i couldnt get this
The enzyme concentration i the experiment is 1nm (1*10^-9M) What is kcat for the uninhibited enzyme?

Thank you so much!

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bio_man

wrote...

Educator

13 years ago

If you look at this image, I think you will be able to deduce which type it is, because the "v" value is smaller for when Prozac is added. This means it's a noncompetitive inhibitor.

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shelby88 Author

wrote...

13 years ago

Is noncompetitive and uncompetitive inhibitor the same?
What do you think I should start x and y axis with...the numbers are so small and some are so scattered...Can you check if I got the x and y's correct?
For the 1st three:
x(1/s): 1,0.3, 0.1, etc
y(1/v): 0.001, 0.0089, 0.0007
y(1/v): 0.004, 0.0018, 0.001

Last but not least, I need help finding the Km for the enzyme with the inhibitor? Any clue?

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