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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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mki mki
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9 years ago
While working at the bench, you forgot to label two tubes of DNA strands. You know the sequences of each DNA strand and that they contain different amounts of G:C base pairs, but you do not know which strand is in which tube. Describe how you can use spectrophotometry to properly label your two tubes.
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9 years ago
Do a melting curve for each of the samples. The sample with the higher Tm will be the sample with the higher %GC content. You can recover your samples by slowly reannealing them once you've determined the Tm.
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