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How should I choose a suitable secondary antibody?

whcaroline

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9 years ago

How should I choose a suitable secondary antibody?

When I do ELISA, how should I choose a suitable secondary antibody? I know that I should consider about the host species of the primary antibody, but I'm not very clear.

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bio_man

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#1

Educator

9 years ago

Here are a few sources for you:

http://www.sigmaaldrich.com/china-mainland/zh/technical-documents/articles/biology/secondary-antibody-selection.html

http://www.abcam.com/secondary-antibodies/secondary-antibody-selection-guide

http://www.abcam.com/protocols/choosing-an-antibody

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