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Cloning- why is alkaline phosphatase used for?

nutcake852

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11 years ago

Cloning- why is alkaline phosphatase used for?

Cloning- why is alkaline phosphatase used for?

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florence5829

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11 years ago

Alkaline Phosphatase (AP) catalyzes the removal of 5´ phosphate groups from DNA, RNA, ribo- and deoxyribonucleoside triphosphates. Since AP-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate. This property can be used to decrease the vector background in cloning strategies.

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