Measuring DNA Damage

It can also be used on prokaryotes, can't it?

umm no "the detection of DNA damage at the level of the individual eukaryotic cell".

No, it really can be used in some prokaryotes

yeah "some"

True, what about the micronucleus test?

hmm haven't heard about that

But, is there a specific reason, or you just want to know?
Here is something I found, although I'm not sure if it's understandable: http://www.ncbi.nlm.nih.gov/pubmed/10698764

There are definitely several ways, and if you want I'll search more about it.

I did come across that method Alexxx. The reason is that I need to determine if protein synthesis and cell viability overall is being effected due to damage in the DNA or other reasons.

Sorry for the useless post but, can you give me all the details about your work?
I'm not sure, but you might be able to use a simpler method rather than a general detection of DNA damage.

I am currently testing for antimicrobial properties of some chemical compounds. For ones which were determined positive (i.e. antimicrobial), I am investigation how cell death is obtained what is causing cell death so I'm investigating several properties of the cell to see if there were any changes. DNA is one of them so I need to determine if before and after treatment there has been any DNA alteration. At first, a general idea of this would be enough (i.e. determine if it is damaged or not); maybe later, if I would have determined that yes there is DNA damage, I may perform PCR to determine at which point is there DNA damage. But for now, it would be enough to determine if DNA has been damaged or not.