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colleen colleen
wrote...
Valued Member
Posts: 17077
12 years ago
You are trying to discover what novel proteins are expressed by a T cell line in response to interaction with a novel antigen associated with HIV. As this is a potential vaccine candidate, you need to see what features of the immune response are initiated by T cells. How would you go about the task? 
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wrote...
Valued Member
12 years ago
Isolate mRNA from the activated T cells. Use reverse transcriptase to create a DNA copy, use DNA polymerase to synthesize the complementary strand, place strand into vector, and place vector into "host" cell (e.g., bacteria).
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