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Which technique below describes Ouchterlony double diffusion?

mazdacx5

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Which technique below describes Ouchterlony double diffusion?

Which technique below describes Ouchterlony double diffusion?

A) Holes are cut in agarose. Antibodies are placed in one well, and a specimen is placed in
the other well. The agarose is placed in an electrical field, and the antibodies and specimen
migrate out of their wells, meeting in the middle, forming a precipitin line.
B) Holes are cut in agarose. Antibodies are placed in one well, and a specimen is placed in
the other well. The antibodies and specimen diffuse out of their wells, meeting in the
middle, forming a precipitin line.
C) Antibodies are added to liquid agarose, and the agarose is allowed to harden. Holes are
cut in agarose, and specimens are placed in the holes. The specimens diffuse out of their
wells, forming precipitin rings.
D) Antibodies are added to liquid agarose, and the agarose is allowed to harden. Holes are
cut in agarose, and specimens are placed in the holes. The agarose is placed in an electrical
field, and the specimens migrate out of their wells, forming precipitin lines.

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