and another question southern blotting, dna sequencing, pcr, and micro-assays

- Southern blotting is a gel-hybridization technique used to detect specific DNA segments from different sources of DNA samples. It includes separation of DNA sequences on gel by electrophoresis, and the binding of specific complementary probe sequences by base pairing.

http://www.ppdictionary.com/tutorials/southern_blot.htm

- In PCR, primers are used to determine the DNA fragment to be amplified by the PCR process. The primers need to match the beginning and the end of the DNA fragment to be amplified. They direct replication towards each other - the extension of one primer by polymerase then becomes the template for the other, leading to an exponential increase in the target segment. Thus, during this stage in the PCR reaction the primers base-pair with their complementary sequences on the Single stranded template DNA generated in the denaturation step.

http://www.ppdictionary.com/tutorials/Polymerase_Chain_Reaction.htm

- For microarrays, check out this video: http://www.ppdictionary.com/tutorials/microarrays.htm

Reply back if you need further assistance,

Bio_man 8)