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Abessesen Abessesen
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Posts: 673
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6 years ago
The correct sequence of procedures in the Southern blotting (hybridization) technique is:
 
  A. hybridization with radioactive probe.
  B. agarose gel electrophoresis and visualize bands.
  C. transfer (blot) to nitrocellulose filter.
  D. digest DNA with restriction nucleases.
  E. expose filter to X-ray film, develop and observe.
   a. B, A, C, E, D
   b. D, C, B, A, E
   c. C, D, B, E, A
   d. D, B, C, A, E
   e. A, B, C, D, E
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Replies
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6 years ago
ANSWER: d
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