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sam1111 sam1111
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9 years ago
if anyone could help with these questions it would be appriciated Slight Smile

Activity 1: Assessing Starch Digestion by Salivary Amylase
1) Which 3 tubes would you compare to determine the effect of pH on amylase activity? What effect does pH have on amylase activity? Explain. (2 mark)
5) Why was a temperature of 37°C used in most of the experiments performed during this simulation? What effect did freezing have on the activity of salivary amylase? (2 marks)
6) Amylase is also produced in the pancreas and enters the duodenum of the small intestine, where it continues to digest carbohydrates. Why is it important to produce more amylase in this region of the GI tract? (2 marks)

Activity 2: Exploring Amylase Substrate Specificity
 2) Based on the results of test tubes 3 and 6, would you expect bacteria to only use amylase to breakdown carbohydrates?
4)If test tube 3 was boiled, would you expect a change in the Benedict’s test result? What about test tube 5? Explain.

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Staff Member
9 years ago
2) Based on the results of test tubes 3 and 6, would you expect bacteria to only use amylase to breakdown carbohydrates?

The bacteria were able to digest the cellulose because they produce the enzyme, cellulase.

5) Why was a temperature of 37°C used in most of the experiments performed during this simulation? What effect did freezing have on the activity of salivary amylase? (2 marks)

The 37°C incubation is significant because it is the same temperature as body temperature so it should be ideal for the enzyme.
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