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Matyler98 Matyler98
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6 years ago
A potentially serious problem with Taq polymerase in PCR is its lack of proofreading ability. Briefly explain why this can be a problem and at which stages during the PCR process this is most and least problematic.
 
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6 years ago
Replication errors are introduced by Taq polymerase as a result of its lack of proofreading. The earlier they are introduced, the greater the number of descendant copies that will also have the error. Therefore, such errors are least problematic when they occur late in the PCR process.
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