In determining the structure of ribozymes, why was it important that ribozyme sequences could be transcribed in vitro?
What will be an ideal response?
What is the main difference between mismatch repair (MMR) in eukaryotes and prokaryotes?
A. Eukaryotes lack a functional Mut H and Dam methylase.
B. Eukaryotes have proteins homologous to MutS and MutH but not MutL.
C. Eukaryotes only repair the lagging strand.
D. Prokaryotic MMR can repair small loops of unpaired nucleotides, whereas eukaryotes cannot.
E. Both use exonucleases to eliminate the mismatched strand but eukaryotes only require 3'-5' exonucleases.
Order the following genomes from smallest to largest.
i. Mus musculus
ii. Homo sapiens
iii. Escherichia coli
iv. Saccharomyces cerevisiae
v. Drosophila melanogaster
A. iii, v, iv, i, ii
B. iii, iv, v, i, ii
C. iv, iii, v, ii, i
D. iv, v, iii, ii, i
E. v, iv, iii, ii, i
Which one of the following is not known to be present in the 3' or 5' untranslated regions of a eukaryotic mRNA?
A. IRE
B. PME
C. upstream open reading frames
D. histone binding sites.
Which of the following protein tags allows for affinity chromatography using Ni2+ as the ligand?
A. glutathione-S-transferase (GST)
B. His tag
C. maltose-binding protein
D. -galactosidase
E. protein A
Quick Reply
