Pyrosequencing is a widely utilized sequencing technique. In this reaction:
A. the generation of pyrophosphate stimulates luciferase to create light.
B. sulfurylase activates luciferase, which then creates light.
C. DNA polymerase adds dideoxynucleotides.
D. apyrase generates pyrophosphate.
E. sulfurylase converts pyrophosphate to ATP.
The ribonucleoprotein, RNase P, catalyzes the:
A. exonucleolytic removal of the 3' end of tRNA molecules.
B. endonucleolytic removal of the 5' end of tRNA molecules.
C. ATP-independent removal of tRNA introns.
D. degradation of the poly(A) tail.
Which bases are most susceptible to hydrolysis of the glycosidic linkage that connects them to the DNA backbone?
A. adenine and guanine
B. adenine and thymine
C. cytosine and guanine
D. cytosine and adenine
E. cytosine, thymine, and uracil
Centromeres are:
A. sequences on the eukaryotic chromosome that function as an attachment point for proteins linking the chromosome to the mitotic spindle.
B. sequences at the ends of the eukaryotic chromosome that add stability to linear DNA sequences.
C. the sites at which replication initiates in bacteria.
D. sequences in the bacterial chromosome at which the kinetochore forms.
E. GC-rich sequences that code for the proteins forming the kinetochore.
Which of the following is a sequencing method that has allowed for miniaturization of the procedure in part by distributing dense clusters of identical DNA on a solid surface?
A. Sanger method
B. automated Sanger method
C. pyrosequencing method
D. Maxam and Gilbert method
E. reversible transfection method
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