Activation of the IFN- globin promoter is an example of a histone code. Of the steps listed, which would be the last step?
A. exposure of the TATA box in promoter
B. acetylation of nucleosomes
C. transport of the nucleosome
D. methylation of nucleosomes
E. phosphorylation of nucleosomes
Transformation of yeast:
A. requires the yeast cells to be made competent using calcium chloride and heat shock.
B. requires adding an enzyme to digest the cell wall, creating a spheroplast.
C. occurs any time free DNA is present; yeast cells are naturally competent.
D. is not stable for fragments greater than 150,000 bp; only shorter YACs are maintained.
E. in yeast spheroplasts occurs naturally and takes up DNA without any additional manipulation.
Which of the following is true of the protein ubiquitination pathway?
A. The ubiquitin carboxyl terminus is covalently linked to the protein targeted for degradation.
B. The ubiquitin is covalently linked to the amino terminus protein targeted for degradation.
C. The protein targeted for degradation is modified by three different enzymes in the pathway.
D. A single molecule of ubiquitin linked to the target protein is sufficient for destruction.
The enzyme adenosine deaminase acting on RNA (ADAR) catalyzes which of the following nucleotide conversions?
A. adenosine to inosine
B. adenosine to cytidine
C. adenosine to guanosine
D. thymidine to uridine
Splicing silencing sites can be located in both introns and exons.
A. True
B. False
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