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Home Q & A Board Biology-Related Homework Help General Biology
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gaminglifex gaminglifex
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4 years ago
The list below contains steps for cloning a gene of interest. They are in random order, and some may be incorrect. Which sequence places the correct stems in the correct order?

1.Transform the DNA into E. coli.
2.Ligate cut genomic DNA fragments and cut plasmid DNA together using DNA ligase.
3.Digest the plasmid cloning vector with the same restriction enzyme.
4.Isolate genomic DNA and digest that DNA with a restriction enzyme.
5.Spread bacterial cells on medium containing lactose and ampicillin and incubate to allow colonies to grow.
6.Spread the bacterial cells on growth medium containing ampicillin and X-gal and incubate to allow colonies to grow.


▸
4, 3, 2, 1, 5


▸
4, 2, 3, 1, 5


▸
4, 2, 3, 1, 6


▸
4, 3, 2, 1, 6
Textbook 
Biology: Exploring the Diversity of Life

Biology: Exploring the Diversity of Life


Edition: 4th
Authors:
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lol987lol987
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4 years ago
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gaminglifex Author
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4 years ago
Correct Slight Smile TY
kriggs62
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Yesterday
Thank you, thank you, thank you!
2ne1_12345
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2 hours ago
Smart ... Thanks!
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