Identify the correct steps and the correct order for cloning a gene of interest. 1. Transform the ...

Identify the correct steps and the correct order for cloning a gene of interest.
  1. Transform the bacteria.
  2. Mix the postrestriction endonuclease gene of interest with the postrestriction endonuclease
  cloning plasmid.
  3. Incubate the combined DNA fragments with the liagase enzyme.
  4. Use restriction endonucleases to cut the gene of interest and the target DNA.
  5. Spread bacteria on medium containing lactose and ampicillin.
  6. Spread bacteria on medium containing X-gal and ampicillin.
 
  a. 4, 2, 3, 1, 6
  b. 4, 3, 2, 1, 5
  c. 4, 3, 2, 1, 6
  d. 4, 2, 3, 1, 5
  e. 2, 4, 1, 5, 3

Question 2

Which of the following choices is an example of a positive feedback mechanism?
 
  a. ceasing exercise when tired
 b. sweating when hot
 c. contractions in childbirth increasing production of oxytocin
 d. eating when hungry and stopping when full