physio 9.0 exercise 12

Post the questions if you have them, I can help you.

Refer to Activity 4: Western Blotting Technique.
Why the HIV Western blot is a more specific test than the indirect ELISA for HIV?

Refer to Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia.
Describe the importance of the washing steps in the direct antibody fluorescence test.
    
Refer to Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA).
Why a patient might test indeterminate?
    
Refer to Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA).
How the direct and indirect ELISA are different?

Refer to Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia.
How would the results be affected if a negative control gave a positive result?
 
Refer to Activity 4: Western Blotting Technique.
Explain the procedure for a patient with an indeterminate HIV Western blot result.
    
Refer to Activity 2: Comparing Samples with Ouchterlony Double Diffusion.
Why does the precipitin line form?
    
Refer to Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia.
Describe how a positive result is detected in this serological test.
    
Refer to Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia.
Explain where the epitope (antigenic determinant) is located
    
Refer to Activity 2: Comparing Samples with Ouchterlony Double Diffusion.
Did you think human serum albumin and bovine serum albumin would have epitopes in common?
    

The Western blot is more specific because you are looking at separated antigens and specifically how they react with antibodies rather than a mixture of antibodies.


Human serum albumin and bovine serum albumin did have partial identity because they had a single spur form. This indicated that they had epitopes in common.

thanks so much!

No problem, any time!