Top Posters
Since Sunday
5
o
5
4
m
4
b
4
x
4
a
4
l
4
t
4
S
4
m
3
s
3
New Topic  
star star
wrote...
Donated
Valued Member
Posts: 981
13 years ago
You are working in a research lab investigating lignin degradation by the bacterium Eatusdetree totalii which was isolated from a rotting piece of birch your supervisor found while strolling in the forest. The genomic sequence of this bacterium has not yet been determined. You found that the addition of a small quantity of hydrogen peroxide induces production of an enzyme which enables the organism to degrade lignin. Zymography (an electrophoretic technique, based on SDS-PAGE) has revealed that a single form of the enzyme is present in cultures grown under solid state fermentation conditions. You go on to purify the enzyme and find that the purified enzyme catalyzes C-C bond cleavage in the side chains of lignin but is unable to do so without added H2O2. When the enzyme preparation is added to pure cellulose under similar reaction conditions, hydrogen peroxide is consumed but to a lesser degree than when lignin is used as substrate. Interestingly, you find that bubbling pure oxygen into the cultures does not result in any enzyme activity against either lignin or cellulose.
Read 1251 times
1 Reply
Replies
Answer accepted by topic starter
bio_manbio_man
wrote...
Educator
Top Poster
Posts: 33224
13 years ago Edited: 13 years ago, bio_man
Sign in or Sign up in seconds to unlock everything for free
This verified answer contains over 350 words.
1

Related Topics

New Topic      
Explore
Post your homework questions and get free online help from our incredible volunteers
  1100 People Browsing
Related Images
  
 100
  
 237
  
 193
Your Opinion
What's your favorite funny biology word?
Votes: 156

Previous poll results: What's your favorite math subject?