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ethinh ethinh
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10 years ago
Plants of a particular species can either have the dominant wild-type phenotype, tall, or the recessive phenotype called dwarf. Genetic analysis has identified the stature gene, and DNA analysis of tall plants yields a DNA fragment of 7.5 kb corresponding to a portion of the gene. The wild-type gene map is illustrated, along with an autoradiograph showing DNA restriction fragments from a normal parental plant (T; lane 1), a tall plant that carries a copy of the mutant allele (T; lane 2), the two DNA fragment patterns observed in tall progeny plants (T; lanes 3 and 4), and the DNA fragment pattern seen in dwarf plants (D; lane 5).

question - Propose two mutational events that could cause the small DNA fragment that represents the mutant allele.

A. The mutant allele is caused by the addition of 4.0 kb of DNA to the left end of the gene.
B. The mutant allele is caused by point mutation(s) in the gene (a nonsense mutation, for example) which also creates a new restriction site exactly 4.0 kb in from the right end of the map.
C. The mutant allele is caused by the deletion of 3.5 kb of DNA from the right end of the gene.
D. The mutant allele is caused by the duplication of the gene into the 4.0 kb fragment of DNA on the left end of the map.
E. The mutant allele is caused by the deletion of 3.5 kb of DNA from the middle of the gene.


link to picture.
http://session.masteringgenetics.com/problemAsset/1511970/8/Sand.ch10.p24.jpg
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wrote...
10 years ago
B. The mutant allele is caused by point mutation(s) in the gene (a nonsense mutation, for example) which also creates a new restriction site exactly 4.0 kb in from the right end of the map.
E. The mutant allele is caused by the deletion of 3.5 kb of DNA from the middle of the gene.
Source  Mastering Genetics
wrote...
9 years ago
In northern blot analysis of mRNA, what mRNA differences would you anticipate for your two proposed mutational mechanisms?
wrote...
Staff Member
9 years ago
deletion would alter size and stature of mRNA
point mutation would NOT alter size and stature of mRNA
Ask another question, I may be able to help!
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9 years ago
About midnight on Saturday, the strangled body of a regular patron of the Seedy Lounge was found in an alleyway near the bar. The police interview of workers and patrons A-R remaining in the bar. A few of the patrons indicate that several individuals, including the bartender, owed money to the victim. The police notice that the bartender and patrons A, C, D, F, K, L, O and R all have recent cuts and scratches on their faces and backs of their necks, but are told that these happened during mud-wrestling matches earlier in the evening. DNA samples are obtained from the bartender and the bar's patrons, from tissues of the victim, and from scrapings of her fingernails. STR analyses are performed on the DNA samples using three of the markers described in Question 10.43: THO1, D18S51, and D21S11. The sizes of the PCR products obtained in each DNA sample for each marker are shown in the table.onsidering the results of only STR marker THO1, which individual(s) should the police investigate further?
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