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Valortim Valortim
wrote...
Posts: 1
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9 years ago
Hi,
I crossed true breeding AB+ females with a balancer chromosome (don't recombine) to true breeding ++c males (A,B,c on same autosomal chromosome) and got F1 apparently heterozygous A,B,+ phenotype. I did a male parental backcross of these F1 males to true breeding ++c females, and got 4 offspring with the unexpected phenotype of A,+,+. This shouldn't happen since male Drosophila don't recombine, and it throws off my data analysis for determining . What could explain this (other than contamination)?

i.e.
AB+//AB+ f  x ++c//++c  m Rightwards Arrow  apparently A,B,+

if [AB+//++c] m  x ++c//++c  f Rightwards Arrow A,B,+ and +,+,c expected and observed, A,+,+ not expected and observed (rarely)


Thanks!
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wrote...
Educator
9 years ago
Hi Valortim,

Could you please give us an update on what you ended up answering?
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