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barry barry
wrote...
Posts: 11630
11 years ago
You discover a mutant strain of Neurospora crassa that will not grow on a minimal medium but that will grow on a medium supplemented by the amino acid methionine. How can you determine which step in the methionine synthesis pathway is affected by the mutation?
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Staff Member
11 years ago
You would grow the strain on a series of media each supplemented with a chemical intermediate in the methionine biosynthetic pathway and observe for growth. The mutant will be able to grow on any medium that contains an intermediate substance in the pathway that comes after the step controlled by its mutant gene. It will also accumulate the intermediate compound used in the step where it is blocked.
- Master of Science in Biology
- Bachelor of Science
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