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irina irina
wrote...
Posts: 919
12 years ago
Does adding a protease enzyme to isolate DNA affect chromosome structure?
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wrote...
12 years ago
Yes it does.  Assuming you are talking about eukaryotic chromosomes, each chromosome is an assemblage of a single long DNA molecules wrapped around many proteins, especially but not only histone proteins.  The protease breaks down these proteins, disrupting the chromosome structure.

You can read more about chromosome structure here http://en.wikipedia.org/wiki/Chromosome
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