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smaapatel smaapatel
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11 years ago
Why is it that when a bacterial gene involved in virulence is knocked out, and then restored via a plasmid, virulence is not completely restored?
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wrote...
11 years ago
This will definitely depend on the species in question.  (Bacillus anthracis' main virulence factors are encoded on plasmids, not the chromosomes.)

The most logical explanation for this phenomena is that the original virulence gene was regulated in a way that maximized the virulence.  There may have been a promoter region near the original site of the gene.  The plasmid may not have this region and therefore may not be expressed as often.
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