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Home Q & A Board Biology-Related Homework Help Biochemistry
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anatomy99 anatomy99
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8 years ago
Can someone explain induction by IPTG

And why DE3 is essential in induction?
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#1
Staff Member
A year ago
IPTG induction is a method of regulating protein synthesis by triggering transcription of the lac operon. It requires two key players: Cells During IPTG induction, cells must produce T7 RNA polymerase required for gene transcription; for example, you can use BL21 (DE3) E. coli strain. BL21 (DE3) has the lacI gene encoding the lac repressor, LacI.
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