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Home Q & A Board Laboratory Help Introductory Courses
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dave67tapout dave67tapout
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11 years ago
I am trying to find a protocol for our teaching lab that takes as little time as possible since we only have class time to do it in. Anyone have a quick transformation protocol?
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#1
11 years ago
Try TOPO cloning. You'll get a kit with a gene fragment, a TOPO vector to ligate the fragment into (takes 5 min) and then you chemically transform the ligated plasmid into E. coli cells by heating the bacteria at 42 degrees celsius for 30 sec. The cells have to grow for about 1 hour and then they can be spread on plates.

Easy and quick.Details in the link below.
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