× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
5
a
5
k
5
c
5
B
5
l
5
C
4
s
4
a
4
t
4
i
4
r
4
Home Q & A Board Biology-Related Homework Help Cell Biology
New Topic  
rkearl27 rkearl27
wrote...
Posts: 165
Rep: 1 0
11 years ago
The cells were washed with PBS, cleaved with trypsin, and media was added and the flask was then placed in an incubator. In order to change the media, do you just have to filter/suction out  the previous media/solution and add another few ml's of media?
Read 437 times
1 Reply

Related Topics

Replies
wrote...
#1
11 years ago
You can just pour off the old media and add the new since the cells will be sticking to the bottom of the tissue culture flask to form a layer.
If you want to split them up or scale up the flask it is best to use a centrifuge to separate the cells from the media.
New Topic      
Quick Reply
Explore
Post your homework questions and get free online help from our incredible volunteers
  1288 People Browsing
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Live Chat
Related Images
  
 1554
  
 738
  
 951
Your Opinion
Which country would you like to visit for its food?
Votes: 204
Previous poll results: Who's your favorite biologist?