Can we culture Peripheral blood in RPMI 1640 MEDIA WITH 40% FOETAL BOVINE SERUM, hOW MUCH IS THE SUCESS RATE C

Well - those conditions *might* cause some white blood cells to grow, but the RBCs are anucleated and will not divide.

Serum contains growth factors that "keep cells happy" and stop them dying by apoptosis; for some cells, it also induces them to grow. The white blood cells might intrepret these conditions as a sort of "pro-inflammatory" signal, which might therefore induce them to multiply. Unless they are transformed cells, however, they will still only divide a limited number of times.

Good luck.

The short answer is no. Unless you're trying to culture circulating fibroblast stem cells (which would require a *lot* of blood), most of the cells in your blood cannot be cultured. White blood cells in your blood do not normally continue to proliferate unless they are cancerous. Red blood cells lack a nucleus. I would suggest using a primary culture of bone marrow tissue, which contains haemopoetic stem cells that give rise to blood cells.

Also, 40% FBS is too high for any type of culture, I imagine cells will grow fine in such high levels of serum, but think of the cost! Most types of cells grow fine in 10~20% serum, and some require even less.