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Home Q & A Board Biology-Related Homework Help Cell Biology
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liely93 liely93
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12 years ago
Some of my isolates have the same sequence as the forward primer in the beginning of the sequence. Is the primers amplified too during PCR that subsequently it can be noticed during the DNA sequencing of the isolates? If i want to proceed with constructing a phylogenetic tree, is it true that the sequence data only valid if i cut off/delete the look-alike primer sequence?

Thank you in advance!
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buhneeduh1218buhneeduh1218
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12 years ago
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