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calculate the concentration of alkaline phosphatase (in units/L)?

flossem

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11 years ago

calculate the concentration of alkaline phosphatase (in units/L)?

calculate the concentration of alkaline phosphatase (in units/L) when 0.5 mL of the enzyme catalyses 450 nmol of p-nitrophenyl phosphate in 10 min

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nursingguy

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#1

11 years ago

One unit will convert one micromole of p-nitrophenyl phosphate to p-nitrophenol and phosphate per minute at 37C in the presence of AMP (2-amino-2-methyl-1-propanol) at pH 10.35.

From your data 1.0 ml enzyme catalyses 900nmole in 10 minutes = 90 nmoles per minute = 0.09 micromoles = 0.09 Units.

Answer accepted by topic starter

nutjob2011

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#2

11 years ago

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