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flossem flossem
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12 years ago
calculate the concentration of alkaline phosphatase (in units/L) when 0.5 mL of the enzyme catalyses 450 nmol of p-nitrophenyl phosphate in 10 min
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12 years ago
One unit will convert one micromole of p-nitrophenyl phosphate to p-nitrophenol and phosphate per minute at 37C in the presence of AMP (2-amino-2-methyl-1-propanol) at pH 10.35.



From your data 1.0 ml enzyme catalyses 900nmole in 10 minutes = 90 nmoles per minute = 0.09 micromoles = 0.09 Units.
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nutjob2011nutjob2011
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12 years ago
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