Molecular Biology

arsenal0312

wrote...

13 years ago

Hi, I'm stuck on this question
A researcher wants to test a new restriction enzyme on the plasmid pAMG22 of 6.3kb. The digest is run in an agarose gel against linear standards. However only a single band was present in the plasmid digest lane and when its size was calculated from the standard curve it was found to be only about 2kb.Explain this result

If you could help with this one i'd appreciate it

thanks

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bio_man

wrote...

Educator

13 years ago

I'm assuming that the restriction enzyme must have "cut" the plasmid in 3 different areas evenly spread throughout the genome (i.e. 6.3 divided by 3 = 2.1 kb).

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