Mitochondrial Research

You'd need to isolate the mitochondria from cells. Any cells will work, even ones taken from a vegetable. You'll need the protocol on how to isolate mitochondria from tissue (attached).

You'll need to find something that changes the mitochondria homogenate a specific color as it produces ATP. I remember doing a lab were the chemical added would change the solution from green to yellow as it was heated, but as soon as we added the inhibitor, it stopped doing this. We had to work really quickly and bathe the test tubes in a warm water bath, and make observations on the color after x amount of minutes. I really wish I had my year two notes on me, this would all make way more sense. Anyway, ask a lab technician or a cell biologist at your school and they'll likely know what I'm talking about.

Thanks for your attention bbb
 Explain me more your theory...How can your theory of color changing will benefit in studying how a specific compound acts within the mitochondria... How can i know if the compound is enabling or stoping electron transport hain for example

 i will thank your answer

That depends on the chemical you use. What did you end up doing for your experiment? Mind sharing

PS sorry for getting back so late.