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tiger_dna tiger_dna
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13 years ago



pO2      YO2   
            
10      0.053833   
20      0.312793   
30      0.605707   
40      0.784544   
50      0.876725   
60      0.924753   
70      0.951256   
80      0.966811   
90      0.976458   
100      0.982728   
         















Plot the saturation curve and the Hill plot and determine from the Hill plot :       
a.    Hill constant  n
b.   p50
c.   K    apparent dissociation constant for the system


Saturation Curve: x-axis= po2, y-axis = Yo2

Hill plot: x-axis =log po2, y-axis = log {Yo2/1-yo2}

log {Yo2/1-yo2}=n log po2-n log p50

From Hill plot, determine n, p50, K

Can someone show me how to do it manually?
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wrote...
#1
Donated
Trusted Member
13 years ago
Do you have any samples?
wrote...
#2
13 years ago
no not really. If I had a solved out solution to a similar problem I would have understood it but unfortunately for me I don't.
wrote...
#3
Educator
13 years ago
Apparently they are supposed to look like this:



From class discussion of how to determine P50 and Hill coefficients for ligand -binding proteins.

But since I haven't done one, I really can't interpret its meaning.
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