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colleen colleen
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10 years ago
Assume that you grew a culture of E. coli for many generations in medium containing 15N (from the ammonium ion), a heavy isotope of nitrogen. You extract DNA from a portion of the culture and determine its density to be 1.723 gm/cm3 (call this sample A). You then wash the remaining
E. coli cells, grow them for one generation in 14N, and extract the DNA from a portion of the culture (call this sample B). You let the culture grow for one more generation in 14N and extract the DNA (call this sample C). Each sample of DNA (A, B, and C) is then subjected to ultracentrifugation. Present the centrifugation profiles that you would expect under (a) semiconservative replication and (b) conservative replication. (Note: Assume that unlabeled [14N] DNA has a density of 1.700 gm/cm3.)
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saviosavio
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10 years ago
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Biology - The only science where multiplication and division mean the same thing.

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colleen Author
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#2
Valued Member
10 years ago
SWEET!!
and251
Sunshine ☀ ☼
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#3
10 years ago
Thanks!!
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#4
10 years ago
Thank you!! This is awesome
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#5
9 years ago
Thank you! This is great
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#6
9 years ago
Is the accompanying figure DNA or RNA? Is the circle closer to the 5' or 3' end?

 
I think it's a DNA because it has and A and T.  I also think it's 5',  because the circle is on the upper left corner.  i can't download the picture. 
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#7
9 years ago
Thank you!
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#8
9 years ago
I need help with this question, cant get the concept right.thank you.
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#9
9 years ago
Thank you, this is so helpful!
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#10
9 years ago
thanks
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#11
9 years ago
Thank you!
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