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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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colleen colleen
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10 years ago
Nucleic acid blotting is commonly used in molecular biology. Two types, Southern blots and Northern blots, involve gel electrophoresis and a filter, which holds the nucleic acid. Briefly describe the procedure of "blotting" in this context and differentiate between Southern and Northern blots. 
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Sunshine ☀ ☼
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10 years ago
In a Southern blot, the DNA to be "probed" is cut with a restriction enzyme(s); then the fragments are separated by gel electrophoresis. Alkali treatment of the gel denatures the DNA, which is then "blotted" onto the filter ( nylon or nitrocellulose). A labeled probe (RNA or DNA) is then hybridized to complementary fragments on the filter. In a Northern blot, RNA is separated in the gel and "probed" with the labeled DNA. 
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Eric Clapton
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colleen Authorcolleen
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