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Home Q & A Board Biology-Related Homework Help Zoology and Botany (Moderator: ehd123)
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|R2r| |R2r|
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Posts: 117
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10 years ago
Hi,

Im doing a really annoying in silico cloning project (using vector NTI software).  DNA and protein technology isnt really my forte (Im rubbish at it) and I was wondering if someone who knows about these things could tell me if Im in the right direction.

Ok so I have to:
 
Design and construct an expression vector based on pGEX-2T
that could be used to express my target protein - FAS2 (arabidopsis thaliana fasciata 2) as a recombinant fusion protein with glutathione S transferase in a bacterial host

Okay now I'm sorry if this is a REALLY stupid question, but my stupid Uni has pretty much piled this assignment on us without any actual teaching  

so does this mean that im going to use pGEX-2T as my vector.......and fuse my FAS2 protein with glutathione S transferase.  so stick that fusion protein into pGEX-2T - and then stick the entire thing in a bacterial host???

Just want to know if Im totally lost and retarded etc.
Thanks for any help anyone can give me!!!!

Cheers
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miaplayer93miaplayer93
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Posts: 40
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10 years ago
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