HOMEWORK MICROBIAL GENETICS Chapters 8,9

ACTIVITY MICROBIAL GENETICS Chapters 8,9 1.What are the base pairs for DNA? 2. A gene is a ______ 3. Chromosome is _______DNA 4. Genes in bacteria can be turned on and off through induction and repression respectively. Bacteria have positive and negative regulation. Eukaryotic cells are different in the ways they turn genes on and off. OPERON STRUCTURE figure 8.12 in textbook How many genes does this operon have? What is I? What does P and O do? What are Z Y and A? INDUCIBLE OPERON OFF figure 8.12 textbook Do you see lactose present? Is operon on or off? Which gene makes the repressive protein? Where does the repressive protein bind? Does the repressive protein allow or prevent making the transcript? INDUCIBLE OPERON ON figure 8.12 textbook Do you see lactose present? Is operon on or off? Which gene makes the repressive protein? Does the repressive protein bind? Why not? Does the repressive protein allow or prevent making the transcript? What enzymes are made at the end? What are they used for? REPRESSIVE OPERON ON figure 8.13 textbook Do you see tryptophan present? Is operon on or off? What enzymes are made at the end? What are they used for? REPRESSIVEE OPERON OFF figure 8.13 textbook Do you see tryptophan present? Is operon on or off? Where does the repressive protein bind? Does the repressive protein allow or prevent making the transcript? In negative regulation (induction or repression) the product of the reaction or the nutrient is available. In positive regulation a nutrient other than the nutrient that the operon deals with appears best and shuts down the lactose enzymes. Bacteria will prefer glucose over lactose. 5. Go to table 9.2 and list 5 therapeutic products of genetic engineering DNA 6. Go to table 9.3 and list 3 agricultural products of recombinant DNA 7. Mention one safety concern and one ethical concern with genetic engineering. Read p. 258-259 8. In research miRNAs and siRNAS are very popular. What are they and what can they be used for? OPERON STRUCTUREINDUCIBLE OPERON REPRESSIVE OPERON